Course organizer: | Dr. Jorge Sztein (NIH, USA). |
Collaborators: | Jesús Martínez Palacios (CIEMAT, Spain) y Marta Treimun (CECs, Chile). |
Date: | 27 January 2012. |
Venue: | Biology Laboratories, Centro de Estudios Científicos (CECs), Valdivia, Chile. |
Duration: | Approximately 3 h. |
Time: | 09:00 to 12:00 h. |
Places: | 15 students. In case of over-subscription there will be a selection procedure. |
Registration deadline: | 15 December 2011. |
Aim: | To learn the mouse sperm cryopreservation method used at Jackson Laboratory. |
Audience: | The course is aimed at all those that are interested in setting up this technique in their own laboratories. |
Course Plan: |
1.-Theoretical introduction and handing out of the Technical Guide. 2.- Demonstration of epididymal dissection. 3.-Sperm cryopreservation carried out by each of the trainees according to the protocols discussed. 4.-Demonstration of cryotube thawing. |
NB: This course will require animal manipulation and the handling of liquid nitrogen. |