Alejandro San Martín Print
Alejandro San Martin was born in Santiago in 1983. At a very early age he moved to live in the southern city of Villarrica, where he studied primary and secondary. He began high school in 2001 at the Universidad Austral de Chile in Valdivia, where studied Medical Technology. His undergraduate thesis was made at the Institute of Biochemistry at the same university, through wich techniques of biology molecular, designed and built a series of chimeric proteins combining the most important domains of hGBF-1 and HBIG-1 (protein play an important role in the processes of formation of secretory vesicles in the cell), with the aim of identifying critical domains for the localization and substrate selectivity. This work was performed under the supervision of Dr. Alejandro Claude. After got the degree from the Medical Technology career, he worked at the hospital in the city of Castro (Chiloé), an experience that finally convinced that their interests were in science and not in the attendance. The year 2008 joined the CECs to work with Dr. Felipe Barros as a research assistant with the intention of creating a line of transgenic mice that express a hexose nanosensor by Cre-Lox system. The year 2009 joined the Ph.D. program in Science and Molecular Cell Biology in the Faculty of Sciences of the Universidad Austral de Chile, as fellowship CONICYT , where he began his formal scientific training under supervision of Dr. Felipe Barros Olmedo. Currently he is working on the development of genetically encoded sensors based on FRET for detection of metabolites and the design of new methods for the study of cancer and diabetes using the same, work who end in 2013 getting the degree of PhD of Science in cellular and molecular biology.
PUBLICACIONES SELECCIONADAS
Imaging Mitochondrial Flux in Single Cells with a FRET Sensor for Pyruvate. PLoS ONE 9(1): e85780. doi:10.1371/journal.pone.0085780
San Martín A, Ceballo S, Baeza-Lehnert F, Lerchundi R, Valdebenito R, Contreras-Baeza Y, Alegría K. and Barros LF. (2014).
Small is fast: astrocytic glucose and lactate metabolism at cellular resolution. Front. Cell. Neurosci. 7:27. doi: 10.3389/fncel.2013.00027
Barros LF, San Martín A, Sotelo-Hitshfeld T, Lerchundi R, Fernández-Moncada I, Ruminot I, Gutiérrez R, Valdebenito R, Ceballo S, Alegría K, Baeza-Lehnert F and Espinoza D (2013).
A Genetically Encoded FRET Lactate Sensor and Its Use To Detect the Warburg Effect in Single Cancer Cells. PLoS ONE 8(2): e57712. doi:10.1371/journal.pone.0057712.
San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., Frommer, W.B., and Barros et al. (2013).
Fast and reversible stimulation of astrocytic glycolysis by K+ and a delayed and persistent effect of glutamate. J Neurosci. 2011 Mar 23;31(12):4709-13.
Bittner CX, Valdebenito R, Ruminot I, Loaiza A, Larenas V, Sotelo-Hitschfeld T, Moldenhauer H, San Martín A, Gutiérrez R, Zambrano M, Barros LF.
Tacrolimus causes a blockage of protein secretion which reinforces its immunosuppressive activity and also explains some of its toxic side-effects
Rauch MC, San Martín A, Ojeda D, Quezada C, Salas M, Cárcamo JG, Yañez AJ, Slebe JC, Claude A. Transpl Immunol. 2009 Dec;22(1-2):72-81
PATENT
Patent application, Genetically encoded probe for quantification of lactate with high spatial and temporal resolution, and methods for quantifying metabolic rates and lactate transport using the same. International application number PCT/US12/33639.
San Martín, A., Ceballo, S., Frommer, W.B. & Barros, L.F. (2012)
Patent application, Genetically encoded probe for quantification of pyruvate concentration with high spatial and temporal resolution, and methods using the same for quantifying pyruvate transport, cellular pyruvate production and consumption, and mitochondrial pyruvate consumption in intact cells. Application number: PCT/IB2013/056766.
San Martín, A., Ceballo, S. & Barros, L.F. (2013)
MEETING PRESENTATIONS
A genetically-encoded FRET sensor for lactate: a new tool for cancer drug discovery. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, & Carnegie Institution of Wahington. Dubai, UAE, 2013. 5th International Conference on Drug Discovery & Therapy.
San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., 3Frommer, W.B. & Barros, L.F.
New methods for the study of lactate metabolism with a genetically encoded FRET nanosensor. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, & Carnegie Institution of Wahington. Bacelona, Spain, 2012. Multidisciplinary approaches to quantify astrocyte neuron signaling.
San Martín, A., Ceballo, S., Ruminot, I., Lerchundi, R., Frommer, W.B. & Barros, L.F.
XXV Annual meeting of the chilean society for cell biology. LACONIC, a genetically encoded FRET nanosensor for lactate. Center for Scientific Studies (CECs), Valdivia, University Austral of Chile, Valdivia, Carnegie Institution of Wahington, 2011.
San Martín, A., Ceballo, S., Frommer, W.B.; Barros, L.F.
XXII Annual meeting of the chilean society for cell biology. “No catalitic region of hGBF-1 are essencial for his localitation and function in the secretory pathway”. Institute of Biochemistry, University Austral of Chile, Valdivia, Chile, 2008.
San Martín, A., Rauch, M.C. y Claude, A.
III International meeting of Farmacology y Therapéutic. Cuban society of Farmacology. Diabetogenic effect of immunosuppressive drugs FK506 by blocking insulin secretion in pancreatic cells. La Habana, Cuba, 2007.
Rauch, M.C., San Martín, A., Ojeda, D., Quezada, C., Salas, M., Cárcamo, J.G., Yánez, A.J., Slebe, J.C. y Claude, A
XX Annual meeting of the chilean society for cell biology. “Análisis de la función de hGBF-1 y hBIG-1 mediante el silenciamiento por siRNA y construcción de proteínas quiméricas”. Institute of Biochemistry, University Austral of Chile, Valdivia, Chile, 2006.
San Martín, A., Rosas, J., Rauch, M.C. y Claude, A.
CONTACT
Telephone : +56-63-234524
Fax : +56-63-234517
E-mail :lc.scec@ojelaa